Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications. Broad reactivity makes this antibody an excellent loading control. A BLAST analysis was used to suggest that this antibody would react with GAPDH from a wide range of organisms, including most vertebrates and some yeast. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Purity/SpecificityĪnti-GAPDH Antibody is directed against human GAPDH protein. This Anti-GAPDH Antibody product is a new product replacing p/n 600-401-A33. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Application NoteĪnti-GAPDH Antibody has been tested for use in ELISA and western blotting. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. A loading control antibody is critical for the correct interpretation of your western blot. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy.
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